| Affiliations: | |
| Project Leader: | Pratima Chennuri, Ph.D. pratimachennuri@tamu.edu Entomology |
| Faculty Mentor: | Kevin Myles, Ph.D. |
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Meeting Times:
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Summer 2017: TBD |
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Team Size:
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6 (Team Full) |
| Open Spots: | 0 |
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Special Opportunities:
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Research experience and guidance from experienced researchers, opportunity to learn methods in recombinant DNA technology such as cloning in addition to using current genome editing technologies such as CRISPR/Cas9-mediated genome engineering. To the enthusiastic and interested scholars, a great opportunity to learn, apply, and even get hired on a long-term basis within the lab. |
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Team Needs:
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Experience of working in a molecular biology or cell culture or Drosophila laboratory preferred. Basic molecular biology laboratory skills, ability and more importantly enthusiasm to learn new techniques and apply them in a timely manner, self-motivated and interested in molecular biology and genetics. |
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Description:
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The project involves engineering DNA constructs, cloning the constructs in to plasmids in E. coli, extraction and purification of plasmid DNA, and their injection in to Drosophila embryos. Scholars will spend some of their time in cloning constructs and the downstream processing of plasmid DNA. Some of the time will be spent on conducting experiments in classical Mendelian fly genetics that will complement the cloning along with associated general fly husbandry and laboratory duties. There might be opportunities for some scholars to test the cloned plasmids in insect cell cultures. |