| Affiliations: | |
| Project Leader: | Rachel Lynch, Ph.D. rlynch@medicine.tamhsc.edu Molecular & Cellular Medicine |
| Faculty Mentor: | David Threadgill, Ph.D. |
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Meeting Times:
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Summer 2016 (complete) |
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Team Size:
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4 (Team Full) |
| Open Spots: | 0 |
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Special Opportunities:
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Team Needs:
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Previous laboratory experience is not required, and all science-related majors are invited to apply. Candidates must be willing to work with mice and take the required mouse handling trainings. |
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Description:
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Somatic mutations in a variety of tumor suppressor genes and oncogenes are associated with progression of colorectal cancer. In this study, we utilize Cre-lox mediated site-directed recombination in mice to induce activation of inactivation of specific genes associated with colon cancer in humans in order to compare the effects of these mutations within the colonic epithelium. Team members will assist in setting up mouse mating crosses to obtain the desired allelic combinations, inject a steroid-derived compound in the mice to initiate recombination at the desired time, assist in local delivery of adeno-viral cre (for those that have completed BSL-2 training), perform colonoscopies, and participate in necropsies of the mice to analyze the resulting colonic epithelium and cancer progression. |