| Affiliations: | Genetics & Genomics Research Leadership |
| Project Leader: | Bin Wu, Ph.D. bin.wu@tamu.edu Biology |
| Faculty Mentor: | Hongmin Qin, Ph.D. |
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Meeting Times:
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TBA |
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Team Size:
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2
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| Open Spots: | 0 |
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Special Opportunities:
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By the end of the project, students are expected to 1) Master Essential Skills in Plant Tissue Culture: Gain hands-on experience in maintaining sterile cultures, optimizing plant growth conditions, and conducting genetic transformation in woody plants. 2) Develop Proficiency in Agrobacterium-Mediated Transformation: Learn the intricacies of using Agrobacterium for gene transfer, including preparing competent cells, co-cultivating plant tissues, and selecting transgenic lines. 3) Enhance Analytical and Problem-Solving Abilities: Engage in experimental design, data collection, and analysis, developing a strong foundation in scientific methodology and critical thinking. 4) Prepare for Advanced Pathways in Scientific Research and Biotechnology: Acquire technical skills and knowledge that will be valuable for future careers in environmental conservation, horticulture, biotechnology, and related fields. |
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Team Needs:
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While a foundational understanding of plant physiology and introductory experience in plant tissue culture are advantageous, they are not mandatory prerequisites for joining our team. Selected candidates will receive comprehensive training to ensure they are well-equipped for the project.
We are on the lookout for new team members who can infuse our group with diversity, energy, and commitment. If you’re passionate about plant breeding and eager to contribute to a dynamic team, we encourage you to apply, regardless of your current level of expertise. |
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Description:
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Crapemyrtle (Lagerstroemia sp.) is a widely admired flowering tree across the United States, valued for its vibrant blooms and ornamental appeal. However, its commercial and aesthetic value is increasingly threatened by arthropod pests, particularly the emerging crapemyrtle bark scale (CMBS; Acanthococcus lagerstroemiae). Among the various species, Lagerstroemia speciosa (L.) Pers. has demonstrated partial resistance to CMBS and other pests, making it a promising candidate for breeding programs. While we appreciate all efforts using conventional breeding approaches to incorporate L. speciosa’s beneficial traits into existing hybrids, we are exploring time-saving methods to overcome some technical challenges and genetic incompatibilities.
This project explores an innovative approach by leveraging tissue culture and molecular breeding techniques to enhance cold hardiness and pest resistance in crapemyrtle species. Building on our previous success in inducing a reliable callus from leaf explants of L. speciosa—a crucial first step towards genetic transformation—we now aim to establish an Agrobacterium-mediated transformation system for this species. The project will focus on optimizing plant growth regulators (PGRs) to facilitate the differentiation of transgenic callus, thereby improving the efficiency of adventitious bud differentiation and overall transformation success. The ultimate goal of this undergraduate research project is to develop a robust and efficient genetic transformation system for crapemyrtle. Achieving this will pave the way for advanced molecular breeding practices, potentially leading to the development of new cultivars with enhanced cold hardiness and resistance to pests like CMBS, thereby ensuring the long-term sustainability and commercial viability of this important genus. |