Aggie Research Programs

Texas A&M University

Spring 2020 – CRISPR Based System to Study Bacterial Flagellar Motor

Project Leader: Kathy Rhee
kathyrhee@tamu.edu
Chemical Engineering
Faculty Mentor:
Dr. Pushkar Lele, Ph.D.
Meeting Times:
TBD
Team Size:
 3 (Team Full)
Open Spots: 0
Special Opportunities:
  • Work with CRISPR systems
  • Hands-on experience with molecular biology in bacteria as well as microscopy
  • Potential co-authorship on a peer-review research manuscript
Team Needs:
The majority of this work is wet lab based requiring either knowledge of or willingness to learn molecular biology techniques. We expect team members to be punctual and be a good team member. Some analysis will be done with Matlab (basic understanding preferred).
Description:
We will use CRISPR/dCas9 system to perturb the bacterial motor proteins. We hope that by knocking down these proteins, we will observe a change in membrane potential. Fluorescence microscopy and real time PCR (qPCR) will be employed.